And Characterization Of Protein Aggregates By
Hydrodynamic size-based separation and characterization of protein
Herein we describe a protocol that uses hollow-fiber flow field-flow fractionation (FFF) coupled with multiangle light scattering (MALS) for hydrodynamic size-based separation and characterization of complex protein aggregates.
Methods for the Characterization of Protein Aggregates
The physicochemical characterization of protein aggregates yields an important contribution to further our understanding on many diseases for which the formation of protein aggregates is one of the pathological hallmarks. On the other hand, bacterial inclusion bodies (IBs) have recently been shown t …
Holographic Characterization of Protein Aggregates
Beyond their reduced therapeutic efficacy, protein aggregates elicit immune responses that result in clinical adverse events. 1 – 6 Detecting, counting, and characterizing …
Studying protein structure and function by native …
The combination of SEC and MS is particularly useful for the detection and characterization of protein aggregates (for example, dimers and trimers) or protein …
Protein aggregation, particle formation, characterization & rheology
The early detection and characterization of protein particles or aggregates — their size, structure, morphology, interactions and rheology in therapeutic protein formulations are critical to reduce safety issues (e.g. immunogenic response in biologics) and to ensure stability and optimized delivery etc. [1], [2], [3], [4].
Studying protein structure and function by native …
Alterations in protein structure may have profound effects on biological function. Analytical techniques that permit characterization of proteins while maintaining their conformational and ...
Protein aggregation: Consequences, mechanism, …
Protein aggregation is affected by temperature via influencing protein-protein interaction, conformational stability, protein/aggregates solubility, protein diffusion rate in …
Detection and characterization of protein aggregates by …
Detection of protein aggregates is needed in the process of protein characterization and during optimization of pharmaceutical formulations. This paper describes a technique, which consists of analysing protein aggregates by fluorescence microscopy after staining with the hydrophobic probe Nile Red.
Higher-Order Structure and Protein Aggregate Characterization …
The complex three-dimensional structure of therapeutic proteins may be affected by the conditions used for production, purification, handling, and storage. Changes to this structure affect product quality and could …
Formation and characterization of chitosan-protein particles …
Hybrid protein-polysaccharide particles were formed by complexation of fractal whey protein aggregates and the cationic polysaccharide chitosan. The f…
Methods for characterization of protein aggregates
In this chapter we present methods for the characterization of protein aggregates as particulate materials relevant to their physicochemical and nanoscale properties.
Analytical Techniques for Structural Characterization of …
This review presents the most common analytical methods for the structural characterization of proteins in solids ( Figure 1 ), their basic principles, and a brief …
Fluorogenic Detection and Characterization of Proteins by Aggregation …
Protein is one of the four most important biomacromolecules in living systems. The detection, quantification, localization, and characterization of proteins is essential for an understanding of biological fundamentals, as well as for the diagnostics and treatment of protein-related diseases. By usin …
Aggregation and Oligomerization Characterization of ß …
The principle of nanopore characterization of protein aggregation is illustrated in Figure1C. The main component of a solid-state nanopore sensing system is a single nanometer pore fabricated in silicon nitride membrane that separates two salt solution-filled chambers, the only connection of which was via the electrolyte solution …
Studying Protein Misfolding and Aggregation by Fluorescence …
Protein misfolding leading to aggregation and amyloid fibril formation has been implicated in a variety of neurodegenerative disorders. Under suitably designed in vitro conditions, intermolecular contacts between polypeptide …
Amyloids, amorphous aggregates and assemblies of …
Amyloid and amorphous aggregates represent the two major categories of aggregates associated with diseases, and although exhibiting distinct features, researchers often treat them as equivalent, which demonstrates the need for more thorough characterization.
Characterization of protein aggregates in cream and …
Characterization of protein aggregates in cream and skimmed human milk after heat and high-pressure pasteurization treatments ... Overall, compared to heat treatment, HHP produced a milder effect on protein aggregation, validating the use of this process to better preserve the native state of HM bioactive proteins. Keywords ...
Methods for the Characterization of Protein Aggregates
The physicochemical characterization of protein aggregates yields an important contribution to further our understanding on many diseases for which the formation of …
Mass Spectrometry-Based Characterization of Protein Aggregates …
Protein aggregation is a common mechanism in multiple neurodegenerative and heart diseases and the accumulation of proteins in aggregates is toxic to cells, causing injury and death. The degree of protein aggregation directly correlates with the severity of the disease. Misfolded proteins present th …
Preparation and characterization of toxic Aβ aggregates …
Nature Protocols - Preparation and characterization of toxic Aβ aggregates for structural and functional studies in Alzheimer's disease research. ... Protein aggregation; Abstract.
Hydrodynamic size-based separation and characterization of …
This technique will advance the biochemical, proteomic and biophysical characterization of molecular-weight aggregates associated with protein mutations, …
Characterization of Protein Aggregates, Silicone Oil …
Protein therapeutic exposure to siliconized surfaces during manufacturing and storage has potential to induce protein aggregation or generate protein-…
Fabrication and characterization of lentil protein gels from …
Heat-induced aggregation and gelation in lentil protein isolate (LPI) were studied over pH levels (pH 2-9), protein concentration (1-13%, w/w), and heating time (0.5-16 h). The LPI gels were formed from both fibrillar and particulate aggregates at pH 2 and 7, respectively. The gels formed from fibri …
Mass Spectrometry-Based Characterization of Protein Aggregates …
Protein aggregation is a common mechanism in multiple neurodegenerative and heart diseases and the accumulation of proteins in aggregates is toxic to cells, causing injury and death. The degree of protein aggregation directly correlates with the severity of the...
Characterization of Small Protein Aggregates and …
Self-association of proteins is important in a variety of processes ranging from acquisition of native quaternary structure (where the association is tightly controlled and proceeds in a highly ordered fashion) to aggregation and amyloidosis. The latter is frequently accompanied (or indeed triggered) by the loss of the native structure, but a …
Fabrication and characterization of lentil protein gels …
Heat-induced aggregation and gelation in lentil protein isolate (LPI) were studied over pH levels (pH 2–9), protein concentration (1–13%, w/w), and heating time (0.5–16 h). The LPI gels were formed from both fibrillar and particulate aggregates at pH 2 and 7, respectively. The gels formed from fibrillar aggr
Detection and Characterization of Small Molecule Interactions …
Neurodegenerative diseases such as Parkinson's and Alzheimer's disease share the pathological hallmark of fibrillar protein aggregates. The specific detection of these protein aggregates by positron emission tomography (PET) in the patient brain can yield valuable information for diagnosis and disea …
Fluorogenic Detection and Characterization of Proteins by Aggregation …
This review summarizes aggregation-induced emission (AIE) fluorescence strategies (see figure) for 1) detection, localization, and quantification of proteins; 2) probing polymer conformational transitions of proteins; 3) characterization of protein–ligand interactions; and 4) evaluation of enzyme activities.
A critical review of methods for size characterization of non …
Although size exclusion chromatography (SEC) has been, and will continue to be, the primary analytical tool for characterization of the content and size distribution of non-particulate aggregates in protein pharmaceuticals, regulatory concerns are driving increased use of alternative and complementary methods such as analytical …
Methods for the Characterization of Protein Aggregates
The physicochemical characterization of protein aggregates yields an important contribution to further our understanding on many diseases for which the formation of protein aggregates is one of the pathological hallmarks. On the other hand, bacterial inclusion bodies...
Characterization of Protein Aggregate Composition
Protein aggregation refers to the clumping together or accumulation of mis-folded proteins. This aggregation can occur during the production, administration, storage, and shipping of therapeutic ...
Advances on Size Exclusion Chromatography and …
Biopharmaceutical drugs are large, complex protein molecules derived from living cells. The characterization of the protein-based products is a complicated and challenging task necessary to guarantee their efficacy and safety. A major concern in manufacturing protein biopharmaceuticals is their propensity to form aggregates. The …
Characterization and implications of host-cell protein aggregates …
In the production of biopharmaceuticals such as monoclonal antibodies (mAbs) and vaccines, the residual amounts of host-cell proteins (HCPs) are among the critical quality attributes. In addition to overall HCP levels, individual HCPs may elude purification, potentially causing issues in product sta …
Strategies for the Assessment of Protein Aggregates in …
Within the European Immunogenicity Platform (EIP) (), the Protein Characterization Subcommittee (EIP-PCS) has been established to discuss and exchange experience of protein characterization in relation to unwanted immunogenicity. In this commentary, we, ...